Publication: Lateral Flow Immunoassay for the Detection of Panton-Valentine Leukocidin in Staphylococcus aureus From Skin and Soft Tissue Infections in the United Arab Emirates
dc.contributor.author | Senok, Abiola | |
dc.contributor.author | Nassar, Rania | |
dc.contributor.author | Celiloglu, Handan | |
dc.date.accessioned | 2022-01-17T04:45:00Z | |
dc.date.available | 2022-01-17T04:45:00Z | |
dc.date.issued | 2021 | |
dc.description.abstract | Introduction: Panton Valentine leukocidin (PVL) is a virulence factor which is associated with methicillin sensitive and resistant Staphylococcus aureus (MSSA/MRSA) causing skin and soft tissue infections (SSTI). This study aimed to evaluate a novel lateral flow immunoassay (LFI) for PVL detection in S. aureus cultures and to describe their genotypic characterization. Methods: The study was carried out from January-August 2020 in Dubai, United Arab Emirates. S. aureus isolates associated with SSTI were tested for PVL detection using LFI. DNA microarray-based assays were used for molecular characterization including detection of pvl genes. Results: One-hundred thirty-five patients with a clinical diagnosis of SSTIs were recruited. Sixty-six patients received antibiotics, mostly beta lactams (n=36) and topical fusidic acid (n=15). One-hundred twenty-nine isolates (MRSA: n=43; MSSA: n=86) were tested by LFI and DNA microarrays. All 76 (58.9%) isolates which were unambiguously negative for the PVL in LFI were negative for pvl genes using the DNA microarray. All the LFI PVL positive isolates (n=53) had pvl genes detected. This translates into 100% each for sensitivity, specificity, positive and negative predictive values for the LFI. The LFI typically takes about 15 min inclusive of a 10 min incubation period. Predominant S. aureus clonal complexes (CC) were CC30 (n=18), CC22 (n=13), CC5 (n=12), CC1 (n=11), CC152 (n=8), CC15 (n=7); CC97 (n=7); CC8 and CC20 (n=6 each). Among MRSA, the proportion of pvlpositives (35/43; 81%) was higher than among MSSA (n/N=18/86; 21%). The fusidic acid resistance gene fusC was detected in 14 MRSA (33%) compared to 8 MSSA (9%). A cocarriage of fusC and pvl genes was present in 7 MRSA and in one MSSA. Conclusion: LFI shows excellent diagnostic accuracy indices for rapid identification of PVL in MSSA/MRSA in a setting with high prevalence of pvl+ve strains. The high occurrence of pvl and fusC genes in MRSA strains causing SSTI is of concern and needs constant surveillance. | en_US |
dc.identifier.other | 204-2021.11 | |
dc.identifier.uri | https://repository.mbru.ac.ae/handle/1/704 | |
dc.language.iso | en | en_US |
dc.subject | Lateral flow immunoassay | en_US |
dc.subject | Staphylococcus aureus | en_US |
dc.subject | Panton Valentine leucocidin | en_US |
dc.subject | Skin and soft tissue infection | en_US |
dc.subject | DNA microarray | en_US |
dc.title | Lateral Flow Immunoassay for the Detection of Panton-Valentine Leukocidin in Staphylococcus aureus From Skin and Soft Tissue Infections in the United Arab Emirates | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | en_US |