Publication:
Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice

dc.contributor.authorJamal, Mohamed
dc.date.accessioned2023-02-16T11:00:29Z
dc.date.available2023-02-16T11:00:29Z
dc.date.issued2022-03
dc.description.abstractAbstract: Human induced pluripotent stem cells (hiPSCs) were differentiated into a specific mesoderm subset characterized by KDR+CD56+APLNR+ (KNA+) expression. KNA+ cells had high clonal proliferative potential and specification into endothelial colony-forming cell (ECFCs) phenotype. KNA+ cells differentiated into perfused blood vessels when implanted subcutaneously into the flank of nonobese diabetic/severe combined immunodeficient mice and when injected into the vitreous of type 2 diabetic mice (db/db mice). Transcriptomic analysis showed that differentiation of hiPSCs derived from diabetics into KNA+ cells was sufficient to change baseline differences in gene expression caused by the diabetic status and reprogram diabetic cells to a pattern similar to KNA+ cells derived from nondiabetic hiPSCs. Proteomic array studies performed on retinas of db/db mice injected with either control or diabetic donor– derived KNA+ cells showed correction of aberrant signaling in db/db retinas toward normal healthy retina. These data provide “proof of principle” that KNA+ cells restore perfusion and correct vascular dysfunction in db/db mice.en_US
dc.identifier.urihttps://repository.mbru.ac.ae/handle/1/1054
dc.language.isoenen_US
dc.subjectmesoderm subseten_US
dc.subjectHuman pluripotenten_US
dc.subjectType 2 diabeticen_US
dc.subjectStem cells amelioratesen_US
dc.titleSpecific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic miceen_US
dc.typeArticleen_US
dspace.entity.typePublicationen_US

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