Publication: Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice
dc.contributor.author | Jamal, Mohamed | |
dc.date.accessioned | 2023-02-16T11:00:29Z | |
dc.date.available | 2023-02-16T11:00:29Z | |
dc.date.issued | 2022-03 | |
dc.description.abstract | Abstract: Human induced pluripotent stem cells (hiPSCs) were differentiated into a specific mesoderm subset characterized by KDR+CD56+APLNR+ (KNA+) expression. KNA+ cells had high clonal proliferative potential and specification into endothelial colony-forming cell (ECFCs) phenotype. KNA+ cells differentiated into perfused blood vessels when implanted subcutaneously into the flank of nonobese diabetic/severe combined immunodeficient mice and when injected into the vitreous of type 2 diabetic mice (db/db mice). Transcriptomic analysis showed that differentiation of hiPSCs derived from diabetics into KNA+ cells was sufficient to change baseline differences in gene expression caused by the diabetic status and reprogram diabetic cells to a pattern similar to KNA+ cells derived from nondiabetic hiPSCs. Proteomic array studies performed on retinas of db/db mice injected with either control or diabetic donor– derived KNA+ cells showed correction of aberrant signaling in db/db retinas toward normal healthy retina. These data provide “proof of principle” that KNA+ cells restore perfusion and correct vascular dysfunction in db/db mice. | en_US |
dc.identifier.uri | https://repository.mbru.ac.ae/handle/1/1054 | |
dc.language.iso | en | en_US |
dc.subject | mesoderm subset | en_US |
dc.subject | Human pluripotent | en_US |
dc.subject | Type 2 diabetic | en_US |
dc.subject | Stem cells ameliorates | en_US |
dc.title | Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | en_US |