El Abed, RashidAlshehhi, AishaHassan Khamis, AmarJamal, Mohamed2023-03-292023-03-292022-08https://repository.mbru.ac.ae/handle/1/1089Introduction: This study aimed to evaluate the dynamic cyclic fatigue resistance of heat treated nickel-titanium (NiTi) files after usage and autoclaving. Methods: Two heat-treated NiTi file systems with tip sizes of #25 (ie, ProTaper Gold F2 [Dentsply Sirona, Charlotte, NC] and TruNatomy Prime [Dentsply Sirona]) were selected. Files of each system were divided into 3 experimental subgroups (n 5 10). For subgroup 1, new files were tested to establish the baseline number of cycles to failure (NCF). For subgroup 2, files underwent canal instrumentation in a simulated J-shaped canal block with 35 of curvature and a 16.5-mm length. The simulated canals were pre-enlarged using stainless steel files #10K and #15K. After instrumentation, the files were tested. For subgroup 3, files underwent canal instrumentation as in subgroup 2 followed by autoclave sterilization and then were tested for cyclic failure. The cyclic fatigue test was performed in dynamic pecking motion under body temperature. The time to fracture was recorded and calculated into NCF. Fracture fragment lengths were measured using a digital microcaliper. Statistical analysis was conducted using the independent t test and multifactorial analysis of variance with 95% significance. Scanning electron microscopy was used to examine the topographic characteristics after cyclic fatigue tests. Results: There were no significant differences between file systems in subgroup 1 using new files (P , .05). Although PG reduced the NCF after use, TN showed a significantly increased NCF (P , .05). However, after sterilization, TN showed a reduced NCF compared with the used one (P , .05). Conclusions: Cyclic loading by usage and heat treatment during sterilization may result in different cyclic fatigue resistance according to the geometry and alloy characteristics. (J Endod 2022;48:1428–1433.)enAutoclave sterilizationCyclic fatigueDynamic fatigue testsheat treatment; nickel-titaniumArticle