Macefield, Vaughan G.2021-05-202021-05-202018-01-08204-2018.16https://repository.mbru.ac.ae/handle/1/252Abstract: Both central command and metaboreflex inputs from contracting muscles increase muscle sympathetic nerve activity (MSNA) to non-contracting muscle during sustainedisometric exercise. We recently showed that MSNA to contracting muscle also increases in an intensity-dependent manner, although whether this can be sustained by the metaboreflex is unknown. MSNA was recorded from the left common peroneal nerve and individual spikes of MSNA extracted from the nerve signal. Eleven subjects performed a series of 4 min dorsiflexions of the left ankle at 10% of maximum voluntary contraction under three conditions: without ischaemia, with 6 min of post-exercise ischaemia, and with ischaemia during and after exercise;these were repeated in the right leg. Compared with pre-contraction values, MSNA to the contracting muscles increased and plateaued in the first minute of contraction (50±18vs.34±10 spikes min−1,P=0.01), returned to pre-contraction levels within 1 min of the contraction ending and was not influenced by ischaemia during or after contraction. Conversely, MSNA to the non-contracting muscles was not different from pre-contraction levels in the first minute of contraction (34±9vs.32±5 spikes min−1,P=0.48), whereas it increased each minute and was significantly greater by the second minute (44±8 spikes min−1,P=0.01).Ischaemia augmented the MSNA response to contraction (63±25 spikes min−1after 4 min,P<0.05) and post-exercise ischaemia (63±27 spikes min−1after 6 min,P<0.01) for the non-contracting muscles only. These findings support our conclusion that the metaboreflex is not expressed in the contracting muscle during sustained static exercise.enMetaboreflexMuscle Sympathetic Nerve ActivityStatic exerciseArticle