Tejani, Tameeza HassanaliMilosevic, Alex2022-01-192022-01-192021304-2021.23https://repository.mbru.ac.ae/handle/1/765Objectives: Layered Double Hydroxide (LDH) is capable of fluoride anion exchange and release. This study investigated the effects of incorporating ZnAl-LDH in commercial and experimental dental varnishes, on fluoride release and re-release after charging in NaF. Methods: Five discs of each material (commercial ClinproXT varnish and experimental light cured resin varnish), with and without 9%ZnAl-LDH were immersed and agitated in deionised water (DW) at 37◦C. A fluoride ion selective electrode measured fluoride release in DW daily over two-weeks. At 3, 6, 9 and 12 days the discs were recharged in 15 ml 0.05 M NaF solution (37◦C) for five minutes followed by immersion in DW. Energy dispersive X-rayspectra (EDS), weight changes and SEMs were performed on discs, before and after each cycle. Results: Evidence of ZnAl-LDH was confirmed by the presence of peaks for zinc and aluminium in EDS spectra. Cumulative fluoride and mean fluoride released between Clinpro, Clinpro + LDH and resin were significantly different (ANOVA, Tukey’s HSD post-hoc test, p< 0.001) except between the resin and resin + LDH. Mean fluoride concentrations differed significantly after every recharge between Clinpro, Clinpro + LDH and resin, but not betweenthe resin and resin + LDH (p < 0.01). The weights and SEMs of the experimental resins +/-LDH, appeared to be stable whilst the weights of Clinpro samples +/−LDH, fluctuated and fragmented. Significance: ClinproXT and experimental resin containing ZnAl-LDH recharged with fluoride did not significantly increase fluoride release compared to the unmodified materials. However, all LDH-F incorporated materials demonstrated fluoride recharging properties which appear beneficial to dentistry and thus further work is required to improve these properties.enLayered double hydroxidesResin modified glass ionomer(RMGIC) varnishExperimental light cured resinVarnishFluoride ion selective electrodeFluoride rechargeReleaseEnergy dispersive X-raySpectroscopyScanning electron microscopyArticle