Moharamzadeh, Keyvan2022-02-072022-02-072021304-2021.30https://repository.mbru.ac.ae/handle/1/800Abstract: As resin-based composites (RBC) replace dental amalgam for environmental reasons, there is a requirement to understand the environmental impact of this alternative dental restorative material. In this study we standardize the simultaneous detection of five monomeric components associated with RBCs using high performance liquid chromatography (HPLC) coupled with solid-phase microextraction (SPME). Factors affecting method performance (detection wavelength, calibration conditions, method sensitivity/accuracy/precision, extraction time/efficiency) are evaluated using standard solutions containing the mixture of TEGDMA, UDMA, Bis-GMA, BPA and HEMA. Detection sensitivity and analytical efficiency of the method is optimized for these compounds using 200 nm detection wavelength, PDMS/DVB fiber and extraction time of 90 min. Analytical accuracy of the HPLC is >95% for all monomers, with precision of 2.3–5.1%. Detection limits under the conditions described are 25 μg/L for HEMA, BPA, UDMA, Bis-GMA, and 100 μg/L for TEGDMA. The extraction time is governed by the largest molecular weight compounds.enDental restorationResin-based compositePlastic pollutionHPLCSPMEArticle